MicroRNA MiR-214 Regulates Ovarian Cancer Cell Stemness by Targeting p53/Nanog

作者：Xu Cheng Xiong; Xu Meng; Tan Lei; Yang Hua; Permuth Wey Jennifer; Kruk Patricia A; Wenham Robert M; Nicosia Santo V; Lancaster Johnathan M; Sellers Thomas A; Cheng Jin Q^*

来源：Journal of Biological Chemistry, 2012, 287(42): 34970-34978.

DOI：10.1074/jbc.M112.374611

Previous studies have shown aberrant expression of miR-214 in human malignancy. Elevated miR-214 is associated with chemoresistance and metastasis. In this study, we identified miR-214 regulation of ovarian cancer stem cell (OCSC) properties by targeting p53/Nanog axis. Enforcing expression of miR-214 increases, whereas knockdown of miR-214 decreases, OCSC population and self-renewal as well as the Nanog level preferentially in wild-type p53 cell lines. Furthermore, we found that p53 is directly repressed by miR-214 and that miR-214 regulates Nanog through p53. Expression of p53 abrogated miR-214-induced OCSC properties. These data suggest the critical role of miR-214 in OCSC via regulation of the p53-Nanog axis and miR-214 as a therapeutic target for ovarian cancer.

• 出版日期2012-10-12