A novel quantitative volumetric spreading index definition and assessment of astrocyte spreading in vitro

作者：Tiryaki Volkan Mujdat^*; Ayres Virginia M; Ahmed Ijaz; Shreiber David I

来源：Cytometry, Part A, 2017, 91(8): 794-799.

DOI：10.1002/cyto.a.23183

• cell morphology
• quantitative cell spreading
• 3D shape descriptor
• immunoreactivity
• confocal laser scanning microscope

A novel quantitative volumetric spreading index (VSI) is defined that depends on the total distance between object voxels and the contact surface plane in three-dimensional (3D) space. The VSI, which ranges from 0 to 1, is rotationally invariant around the z-axis. VSI can be used to quantify the degree of individual cell spreading, which is important for analysis of cell interactions with their environment. The VSIs of astrocytes cultured on a nanofibrillar surface and three different comparative planar surfaces have been calculated from confocal laser scanning microscope z-series images, and the effects of both culture surface and immunoreactivity on the degree of cell spreading were investigated. VSI calculations indicated a statistical correlation between increased reactivity, based on immunolabeling for glial fibrillary acidic protein, and decreased cell spreading. Further results provided a quantitative measure for the increased spreading of quiescent-like and reactive-like astrocytes on planar substrates functionalized with poly-l-lysine.

• 出版日期2017-8
• 单位rutgers