Antibody and DNA dual-labeled gold nanoparticles: Stability and reactivity

作者：Qiao, Fei-Yan; Liu, Jun; Li, Fu-Rong; Kong, Xiao-Li; Zhang, Hao-Li^*; Zhou, Han-Xin

来源：Applied Surface Science, 2008, 254(10): 2941-2946.

DOI：10.1016/j.apsusc.2007.10.046

• gold nanoparticle
• self-assembly
• DNA
• antibody

Gold nanoparticles labeled by both antibody (IgG) and single stranded DNA (ss-DNA) have been synthesized and characterized. The stability and reactivity of the dual-labeled nanoparticles were compared with the conventional IgG or ss-DNA modified nanoparticles. It was found that the IgG adsorption significantly improved the stability of the nanoparticles in aqueous solution, which is beneficial for attaching ss-DNA. The presence of IgG also effectively prohibits the desorption of ss-DNA against dithiothreitol (DTT) displacement. The coverage on dual-labeled nanoparticles was found to be 50 +/- 15 ss-DNA/nanoparticle and 10 +/- 2 IgG/nanoparticle, respectively, compared to the value of 70 +/- 15 ss-DNA/nanoparticle of only ss-DNA-labeled gold nanoparticles. Dot-immuno and cross-linking experiments confirmed that both the IgG and ss-DNA retained their bioactivity on the nanoparticle surface. The dual-labeled nanoparticles have potential to be used as novel bio-probes for ultrasensitive detection.

• 出版日期2008-3-15
• 单位兰州大学