Toll like receptor 5 ligand induces monocyte chemoattractant protein-1 in mouse osteoblastic cells

作者：Nakamura Kimiya; Deyama Yoshiaki^*; Yoshimura Yoshitaka; Suzuki Kuniaki; Morita Manabu

来源：Biomedical Research-Tokyo, 2012, 33(1): 39-44.

DOI：10.2220/biomedres.33.39

Flagellin, the ligand of Toll like receptor 5, is the major subunit of bacterial flagella. Flagellin stimulates various cells to release chemokines. Monocyte chemoattractant protein-1 (MCP-1) is a member of the CC chemokine family that is involved in monocyte infiltration in inflammatory diseases. It has been reported that serum MCP-1 levels increase proportionally with the severity of periodontal disease. Inflammatory mediators induce MCP-1 production in various cells, including osteoblasts. However, it remains unclear whether MCP-1 is released from osteoblasts in response to flagellin. In the present study, we investigated the effects of flagellin on the expression of MCP-1 in the mouse osteoblastic cell line, MC3T3-E1 (El) cells. Flagellin markedly increased MCP-1 mRNA level in a dose-dependent manner. The effect of flagellin on MCP-1 mRNA expression in El cells was transient, with a peak at 1 h. Concomitant with MCP-1 mRNA expression, MCP-1 protein levels were clearly elevated at 3 h after flagellin exposure. In addition, we revealed that INK and MEK-ERK1/2 are involved in flagellin-induced MCP-1 expression in El cells. These results indicated that bacterial flagellin may play an important role in the progression of periodontitis. Results of further studies will provide more clues to the prevention of periodontal diseases.

• 出版日期2012-2