AIEOP-BFM consensus guidelines 2016 for flow cytometric immunophenotyping of Pediatric acute lymphoblastic leukemia

作者：Dworzak Michael N.^*; Buldini Barbara; Gaipa Giuseppe; Ratei Richard; Hrusak Ondrej; Luria Drorit; Rosenthal Eti; Bourquin Jean-Pierre; Sartor Mary; Schumich Angela; Karawajew Leonid; Mejstrikova Ester; Maglia Oscar; Mann Georg; Ludwig Wolf-Dieter; Biondi Andrea; Schrappe Martin; Basso Giuseppe

来源：Cytometry Part B: Clinical Cytometry , 2018, 94(1): 82-93.

DOI：10.1002/cyto.b.21518

• standardization
• flow cytometry
• leukemia
• pediatric
• immunophenotyping

Immunophenotyping by flow cytometry (FCM) is a worldwide mainstay in leukemia diagnostics. For concordant multicentric application, however, a gap exists between available classification systems, technologic standardization, and clinical needs. The AIEOP-BFM consortium induced an extensive standardization and validation effort between its nine national reference laboratories collaborating in immunophenotyping of pediatric acute lymphoblastic leukemia (ALL). We elaborated common guidelines which take advantage of the possibilities of multi-color FCM: marker panel requirements, immunological blast gating, in-sample controls, tri-partite antigen expression rating (negative vs. weak or strong positive) with capturing of blast cell heterogeneities and subclone formation, refined ALL subclassification, and a dominant lineage assignment algorithm able to distinguish simple from bilineal/complex mixed phenotype acute leukemia (MPAL) cases, which is essential for choice of treatment. These guidelines are a first step toward necessary inter-laboratory standardization of pediatric leukemia immunophenotyping for a concordant multicentric application.

• 出版日期2018-1