Antibacterial efficacy of rifampin loaded solid lipid nanoparticles against Staphylococcus epidermidis biofilm

作者：Bazzaz Bibi Sedigheh Fazly; Khameneh Bahman; Zarei Hamed; Golmohammadzadeh Shiva^*

来源：Microbial Pathogenesis, 2016, 93: 137-144.

DOI：10.1016/j.micpath.2015.11.031

• Bacterial biofilm
• Bacterial count
• Crystal violet
• Rifampin
• Solid lipid nanoparticles
• Staphylococcus epidermidis

Objective: The aim of the present study was to assess the in vitro anti-biofilm activities of solid lipid nanoparticles (SLN) loaded with rifampin against biofilm-producing Staphylococcus epidermidis.
Methods: SLN were prepared and characterized for size, zeta potentials and encapsulation efficacy. The morphological and thermal properties of formulation were evaluated by TEM imagining and DSC analysis. The anti-biofilm activity of different formulations was assessed at different incubation times and concentrations by crystal violet (CV) and viable biofilm count methods.
Results: The zeta potentials, particle sizes and encapsulation efficiencies of final formulations were 17 +/- 0.7 mV, 101 +/- 4.7 nm and approximately 70%; respectively. Rifampin-SLN was able to reduce the biomass of biofilm at time- and concentration-dependent manner. According to biofilm count results, the Rifampin-SLN was more effective for removal of the bacteria with respect to free rifampin.
Conclusion: The results of this study highlight the advantages and efficiency of Rifampin-SLN in biofilm eradication.

• 出版日期2016-4