Analysis of cocaine and two metabolites in dried blood spots by liquid chromatography with fluorescence detection A novel test for cocaine and alcohol intake

作者：Mercolini Laura; Mandrioli Roberto; Gerra Gilberto; Raggi Maria Augusta^*

来源：Journal of Chromatography A, 2010, 1217(46): 7242-7248.

DOI：10.1016/j.chroma.2010.09.037

• Cocaine
• Metabolites
• Liquid chromatography with fluorescence
• detection
• Dried blood spots (DBS)
• Plasma

An original HPLC method coupled to spectrofluorimetric detection is presented for the simultaneous analysis in dried blood spots (DBS) of cocaine and two important metabolites namely benzoylecgonine (Its main metabolite) and cocaethylene (the active metabolite formed in the presence of ethanol) The chromatographic analysis was carried out on a C8 column using a mobile phase containing phosphate buffer (pH 3 0)-acetonitrile (85 15 v/v) Native analyte fluorescence was monitored at 315 nm while exciting at 230 nm A fast and feasible sample pre-treatment was implemented by solvent extraction obtaining good extraction yields (>91%) and satisfactory precision values (RSD<4 8%) The method was successfully applied to DBS samples collected from some cocaine users both with and without concomitant ethanol intake The results were in good agreement with those obtained from plasma samples subjected to an original solid-phase extraction procedure on C8 cartridges The method has demonstrated to be suitable for the monitoring of cocaine/ethanol use by means of DBS or plasma tes

• 出版日期2010-11-12