Assessment of the cytotoxicity of aluminium oxide nanoparticles on selected mammalian cells

作者：Radziun E^*; Wilczynska J Dudkiewicz; Ksiazek I; Nowak K; Anuszewska E L; Kunicki A; Olszyna A; Zabkowski T

来源：Toxicology in Vitro, 2011, 25(8): 1694-1700.

DOI：10.1016/j.tiv.2011.07.010

• Aluminium oxide nanoparticles
• Cytotoxicity
• Mammalian cells
• L929-murine fibroblast cell line
• BJ - human fibroblast cell line

The rapid development of nanotechnology raises both enthusiasm and anxiety among researchers, which is related to the safety use of the manufactured materials. Thus, the aim of this study was to investigate the effect of aluminium oxide nanoparticles on the viability of selected mammalian cells in vitro. The aluminium oxide nanoparticles were characterised using SEM and BET analyses. Based on Zeta (0 potential measurements and particle size distribution, the tested suspensions of aluminium oxide nanoparticles in water and nutrient solutions with or without FBS were classified as unstable. Cell viability, the degree of apoptosis induction and nanoparticles internalization into the cells were assessed after 24 h of cell exposure to Al2O3 nanoparticles. Our results confirm the ability of aluminium oxide nanoparticles to penetrate through the membranes of L929 and BJ cells. Despite this, there was no significant increase in apoptosis or decrease in cell viability observed, suggesting that aluminium oxide nanoparticles in the tested range of concentrations has no cytotoxic effects on the selected mammalian cells.

• 出版日期2011-12
• 单位河北医科大学