A simple and fast method to analyze the orientation of c-type cytochromes in the outer membrane of Gram-negative bacteria

作者：Richter Katrin; Buecking Clemens; Schicklberger Marcus; Gescher Johannes^*

来源：Journal of Microbiological Methods, 2010, 82(2): 184-186.

DOI：10.1016/j.mimet.2010.04.011

• Outer membrane cytochromes
• Localization
• Riboflavin

Outer membrane cytochromes catalyze the final reduction step of respiratory chains to electron acceptors that cannot diffuse through the outer membrane of Gram-negative bacteria. We developed an in vivo method to detect the orientation of outer membrane cytochromes via analysis of electron transfer reactions between these enzymes and riboflavin.

• 出版日期2010-8