Application of the Alamar blue assay to determine the susceptibility to anti-tuberculosis pharmaceuticals

作者：de Jesus Alba Romero Jose; Luis Ortega Sanchez Jose; Luna Herrera Julieta; Urtiz Estrada Norma; Cervantes Flores Maribel; Ruiz Baca Estela; Ramon Hernandez Salgado Jose; Estela Garcia Perez Blanca; Ruiz Flores Pablo; Martinez Romero Aurora^*

来源：African Journal of Microbiology Research, 2011, 5(26): 4659-4666.

DOI：10.5897/AJMR11.863

• Tuberculosis
• Alamar blue
• drug resistance

The aim of this study was to apply the Alamar Blue assay to determine the susceptibility to anti-tuberculosis pharmaceuticals, in Mycobacterium tuberculosis isolated strains from patients with tuberculosis (TB). In this study, a TB diagnostic was done by bacilluscope as an essential diagnostic tool for pulmonary TB using the Ziehl-Neelsen (ZN) stain. Furthermore, a culture was prepared in Lowenstein-Jensen (L-J) solid media, Middlebrook 7H9, and Middlebrook 7H10. For all the clinical samples investigated, five reference strains of M. tuberculosis were used to standardize the sensibility to streptomycin (SM), isoniazid (INH), rifampicin (RIF), and ethambutol (EMB) of the ATCC. Additionally, 97 Mycobacterium strains were analyzed from clinical isolations. The microplate Alamar Blue assay (MABA) was standardized and applied. The resistance levels of this disease were obtained in La Comarca Region (Lagunera). The results indicated that the MABA test is fast and easy to apply, however the most important aspect is the reliability of the method to determine the drug sensibility to pharmaceuticals. The MABA colorimetric test can be used in different regional as well as state TB diagnostic laboratories.

• 出版日期2011-11-16