Universal correction of enzymatic sequence bias reveals molecular signatures of protein/DNA interactions

作者：Martins Andre L.; Walavalkar Ninad M.; Anderson Warren D.; Zang C*******; Guertin Michael J.^*

来源：Nucleic Acids Research, 2018, 46(2): e9.

DOI：10.1093/nar/gkx1053

Coupling molecular biology to high-throughput sequencing has revolutionized the study of biology. Molecular genomics techniques are continually refined to provide higher resolution mapping of nucleic acid interactions and structure. Sequence preferences of enzymes can interfere with the accurate interpretation of these data. We developed seqOutBias to characterize enzymatic sequence bias from experimental data and scale individual sequence reads to correct intrinsic enzymatic sequence biases. SeqOutBias efficiently corrects DNase-seq, TACh-seq, ATAC-seq, MNase-seq and PRO-seq data. We show that seqOutBias correction facilitates identification of true molecular signatures resulting from transcription factors and RNA polymerase interacting with DNA.

• 出版日期2018-1-25