Anti-Inflammatory and Neuroprotective Constituents from the Peels of Citrus grandis

作者：Kuo Ping Chung; Liao Yu Ren; Hung Hsin Yi; Chuang Chia Wei; Hwang Tsong Long; Huang Shiow Chyn; Shiao Young Ji; Kuo Daih Huang; Wu Tian Shung^*

来源：Molecules, 2017, 22(6): 967.

DOI：10.3390/molecules22060967

• Citrus grandis
• chromatographic method
• spectroscopic elucidation
• superoxide anion generation
• elastase release
• neuroprotective

A series of chromatographic separations performed on the ethanol extracts of the peels of Citrus grandis has led to the characterization of forty compounds, including seventeen coumarins, eight flavonoids, two triterpenoids, four benzenoids, two steroids, one lignan, one amide, and five other compounds, respectively. The chemical structures of the purified constituents were identified on the basis of spectroscopic elucidation, including 1D- and 2D-NMR, UV, IR, and mass spectrometric analysis. Most of the isolated compounds were examined for their inhibition of superoxide anion generation and elastase release by human neutrophils. Among the isolates, isomeranzin (3), 17,18-dihydroxybergamottin (12), epoxybergamottin (13), rhoifolin (19), vitexicarpin (22) and 4-hydroxybenzaldehyde (29) displayed the most significant inhibition of superoxide anion generation and elastase release with IC50 values ranged from 0.54 to 7.57 mu M, and 0.43 to 4.33 mu M, respectively. In addition, 7-hydroxy-8-(2'-hydroxy-3'-methylbut-3'-enyl)coumarin (8) and 17,18-dihydroxybergamottin (12) also exhibited the protection of neurons against A beta-mediated neurotoxicity at 50 mu M.

• 出版日期2017-6
• 单位长春大学