A randomized controlled cross-over study of the effect of alcohol-free chlorhexidine and essential oils on interleukin-1 levels in crevicular fluid

作者：Ramberg Per^*; Albertsson Katarina Wiken; Derks Jan; van Dijken Jan

来源：Swedish Dental Journal, 2016, 40(2): 143-152.

• Clinical
• chlorhexidine
• crevicular fluid
• cytokines
• essential oils
• mouth rinse

Aim of the study was to determine the effect of an alcohol-free chlorhexidine mouth rinse and an essential oils containing mouth rinse on pro-inflammatory cytokine levels in gingival crevicular fluid in participants who continue to perform their regular mechanical oral hygiene regimes and normal dietary practice.
Twenty adult volunteers (mean age: 59 years) participated in the double-blind randomised controlled cross-over study. Three mouth rinses were used during 16-day periods as an adjunctive to regular mechanical oral hygiene: a solution with alcohol-free chlorhexidine (CHX; Paroex (R)), a solution with essential oils (EO; Listerine (R)), and water (negative control).The mouth rinse periods were separated by 3-month washout periods.
At days o (baseline) and 17 (end) of each mouth rinse period, gingival crevicular fluid (GCF) was collected at different tooth sites and analyzed with ELISA technique for IL-1 alpha, IL-1 beta and IL-1ra levels.
No significant correlations between clinical parameters (OHI and GI) and cytokine concentrations were observed, regardless of mouth rinse regimen.The generalized linear models revealed that none of the mouth rinses had a statistically significant impact on IL-1 concentrations in GCF. Large inter-individual variations were observed for the levels of IL-1 alpha, IL-1 beta and IL-1ra. Also the changes in concentrations between day o and day17 for the mouthrinses showed large intra-individual variations.
It can be concluded that neither the alcohol-free chlorhexidine nor the mouth rinse containing essential oils reduced the levels of IL-1 alpha, IL-1 beta and IL-1ra in GCF.

• 出版日期2016