alpha-Actinin interacts with rapsyn in agrin-stimulated AChR clustering

作者：Dobbins G Clement; Luo Shiwen; Yang Zhihua; Xiong Wen C; Mei Lin^*

来源：Molecular Brain, 2008, 1(1): 18.

DOI：10.1186/1756-6606-1-18

AChR is concentrated at the postjunctional membrane at the neuromuscular junction. However, the underlying mechanism is unclear. We show that alpha-actinin, a protein known to cross-link F-actin, interacts with rapsyn, a scaffold protein essential for neuromuscular junction formation. alpha-Actinin, rapsyn, and surface AChR form a ternary complex. Moreover, the rapsyn-alpha-actinin interaction is increased by agrin, a factor known to stimulate AChR clustering. Downregulation of a-actinin expression inhibits agrin-mediated AChR clustering. Furthermore, the rapsyn-alpha-actinin interaction can be disrupted by inhibiting Abl and by cholinergic stimulation. Together these results indicate a role for alpha-actinin in AChR clustering.

• 出版日期2008