A Time-Dose Model to Quantify the Antioxidant Responses of the Oxidative Hemolysis Inhibition Assay (OxHLIA) and Its Extension to Evaluate Other Hemolytic Effectors

作者：Prieto M A^*; Vazquez J A

来源：Biomed Research International, 2014, 2014: 632971.

DOI：10.1155/2014/632971

The development of a convenient mathematical application for testing the antioxidant potential of standard and novel therapeutic agents is essential for the research community to perform evaluations in a more precise form. The in vitro oxidative hemolysis inhibition assay, despite its relevance for in vivo responses, lacks a proper mathematical model to quantify the responses. In this work, a simple nonlinear time-dose tool to test the effectiveness of antioxidant compounds is presented. Themodel was verifiedwith available experimental data fromthe bibliography. Themodel helps to describe accurately the antioxidant response as a function of time and dose allowing comparisons between compounds. Its advantages are a simple application, provision of parametric estimates that characterize the response, simplification of the protocol, economization of experimental effort, and facilitation of rigorous comparisons among the effects of different compounds and experimental approaches. Finally, other effectors that may obstruct or be of interest for the antioxidant determination are alsomodeled in similar principles. Thus, the basis ofmore complexmultivariable models is provided. In all experimental data fitted, the calculated parameterswere always statistically significant, the equations prove to be consistent, and the correlation coefficient of determination was in all cases higher than 0.98.

• 出版日期2014