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摘要
A specific ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method was established and validated for the simultaneous quantification of paclitaxel prodrug (PP) and paclitaxel (PT) in human plasma. PP, PT and an internal standard C-13(6)-labelled PT (C-13(6)-PT) were extracted from plasma by tert-butyl methyl ether and separated on an ACQUITY UPLC BEH C-18 column (50 mm x 2.1 mm x 1.7 mu m) using a mobile phase of methanol-acetonitrile 50: 50 (v/v) at a flow rate of 0.4 mL/min. At positive electrospray ionization mode, multiple reaction monitoring of the precursor-product ion transitions of m/z 882.2 -> 313.9 for C-13(6)-PT, 876.2 -> 307.9 for PT and 1216.5 -> 647.8 for PP was used for the quantification. The linear calibration curve was obtained in a concentration range of 1-1000 ng/mL with a lower limit of quantification of 1 ng/mL. For both analytes, the values of intra- and inter-day precision were within 12.5% and accuracy fell in the ranges of 91.5-103.4%. The recovery ranged from 88.1% to 94.2% and the matrix effects from 89.3% to 95.4%. PP and PT were stable under short-term temperature and post-preparative conditions. The method was applied to stability of PP in human plasma and released PT from PP.
- 出版日期2013-5
