Direct Electron Transfer of Hemoglobin in a Hydrophilic Ionic Liquid/Gellan Gum Composite Film Modified Carbon Ionic Liquid Electrode

作者：Qiao, Li-Fang; Zheng, Jian-Bin^*; Gao, Rui-Fang; Sheng, Qing-Lin

来源：Journal of the Chinese Chemical Society, 2010, 57(4A): 718-725.

DOI：10.1002/jccs.201000100

• Direct electrochemistry
• Gellan gum
• Carbon ionic liquid electrode
• Hemoglobin
• Room temperature ionic liquids

A gellan gum (GG)-Hemoglobin (Hb)-1-butyl-3-methyl-imidazolium tetrafluoroborate ([BMIM] [BF(4)])/carbon ionic liquid electrode(CILE), GG-Hb-[BMIM][BF(4)]/CILE, was fabricated, and the direct electron transfer of hemoglobin and electrocatalytic reaction for electrocatalytic abilities were carefully investigated. Scanning electron microscopy images of the modified electrode showed that it had a dendritic structure. UV-Vis spectra and FT-IR spectra showed that Hb in the composite film retained its native structure. Cyclic voltammogram of the modified electrode showed that a pair of well-defined redox peaks of Hb is observed with the formal potential (E(0)') of -0.278 V (nu s. SCE). The electron transfer coefficient (alpha) and the apparent heterogeneous electron transfer rate constant (k(s)) was 0.44 and 2.0 s(-1), respectively. The modified electrode also exhibited excellent electrocatalytic ability to the reduction of trichloroacetic acid and hydrogen peroxide with a detection limit of 6.8 mu mol/L and 1.5 mu mol/L (S/N = 3), respectively. The apparent Michaelis-Menten constant (K(M)(app)) was estimated to be 4.3 mmol/L and 0.13 mmol/L, respectively. The present study proved that the combination of GG-[BMIM][BF(4)] film with CILE is able to open up new opportunities for the design of enzymatic biosensors with potential applications in practice.

• 出版日期2010-8
• 单位西北大学