Active Langmuir-Schaefer films of tyrosinase-Characteristic

作者：Cabaj Joanna; Soloducho Jadwiga^*; Swist Agnieszka

来源：Sensors and Actuators B: Chemical , 2010, 150(2): 505-512.

DOI：10.1016/j.snb.2010.09.008

• Langmuir-Schaefer films
• Tyrosinase
• Conducting thianthrenes
• Biosensing effect
• AFM microphotography

The structure and biosensor characteristic of complex between phenoloxidase (tyrosinase) and phoshpholipid thin ordered layer were investigated The advantageous of immobilization method based on Langmuir-Schaefer (LS) film as a matrix has been developed for characterization of sensing protein layers effect Tyrosinase from Agaricus bisporus molecules were densely adsorbed onto Langmuir monolayer surface and then deposited on solid support which can be used for phenolic compounds determination The enzyme was immobilized by adsorption process Phenoloxidase was adsorbed on monolayer built of 1 2-dipalmitoyl-S N-glicero-3-phosphocholine (DPGPC) The sensor sensitization was achieved by an amphiphilic conducting structure - N-hexadecy1-3 6-bis(thianthrene)carbazole (HBTC) admixed into the film The interlaced compound was expected to facilitate the electron transfer as well enhancing the sensor sensitivity Considering the fact that immobilization strategy showed high efficiency

• 出版日期2010-10-28