alpha-Glucosidase Inhibitory Effect and Simultaneous Quantification of Three Major Flavonoid Glycosides in Microctis folium

作者：Chen, Yan-Gan; Li, Ping; Li, Peng; Yan, Ru; Zhang, Xiao-Qi; Wang, Ying^*; Zhang, Xian-Tao; Ye, Wen-Cai; Zhang, Qing-Wen

来源：Molecules, 2013, 18(4): 4221-4232.

DOI：10.3390/molecules18044221

• Microctis Folium
• flavonoid glycosides
• alpha-glucosidase inhibitory effect
• HPLC-DAD
• quantification

Microctis Folium, the leaves of Microcos paniculata L., is a commonly used herbal tea material. The methanol extract of Microctis Folium and its principle compounds vitexin (1), isovitexin (2) and isorhamnetin 3-O-beta-D-rutinoside (3) were investigated for their alpha-glucosidase inhibitory effects. The extract showed strong alpha-glucosidase inhibitory effect (IC50 = 61.30 mu g/mL) and the three flavonoid glycosides 1-3 exerted satisfactory alpha-glucosidase inhibitory effects, with IC50 values of 244.0 mu M, 266.2 mu M and 275.4 mu M, respectively. A simple and reliable HPLC-DAD method was developed for the quantification of the three flavonoid glycosides in Microctis Folium and applied successfully to determine contents of these components in samples collected from different locations. This study suggested that Microctis Folium may be a promising candidate for development of herbal antidiabetes drugs, and vitexin, isovitexin and isorhamnetin 3-O-beta-D-rutinoside can be the biomarkers and chemical markers for this plant substance.

• 出版日期2013-4
• 单位澳门大学; 暨南大学; 广东省中医研究所