An electrogenerated chemiluminescent biosensor based on a g-C3N4-hemin nanocomposite and hollow gold nanoparticles for the detection of lactate

作者：Chen Hongmei; Tan Xingrong; Zhang Juanjuan; Lu Qiyi; Ou Xin; Ruo Yuan^*; Chen Shihong

来源：RSC Advances, 2014, 4(106): 61759-61766.

DOI：10.1039/c4ra09616a

In this article, a new electrochemiluminescent (ECL) biosensor based on a g-C3N4-hemin nanocomposite and hollow gold nanoparticles (HGNPs) was constructed to detect lactate. Firstly, the g-C3N4 nanosheets were prepared through ultrasonication-assisted liquid exfoliation of bulk g-C3N4, which was obtained through polymerizing melamine under 600 degrees C. Then, the nanocomposites of g-C3N4 nanosheets and hemin were prepared to modify a glassy carbon electrode. Subsequently, HGNPs were self-assembled onto the electrode for adsorbing lactate oxidase to achieve a lactate biosensor. Due to the excellent catalytic effect of g-C3N4-hemin and HGNPs on the luminol/H2O2 ECL system, the as-prepared biosensor exhibited a good response performance to lactate with a linear range of 1.7 x 10(-8) to 5.0 x 10(-4) M and a detection limit of 5.5 x 10(-9) M. In addition, the prepared ECL biosensor exhibited satisfying reproducibility and stability. The g-C3N4-hemin nanocomposite might have great potential application in a luminol/H2O2 ECL system.

• 出版日期2014
• 单位西南大学