Upregulation of microRNA-155 inhibits trophoblast differentiation through the Wnt/β-catenin pathway

作者：Duan, Ran; Li, Qin; Qi, Hongbo^*

来源：International Journal of Clinical and Experimental Medicine, 2018, 11(6): 5750-5756.

• Pre-eclampsia
• miR-155
• calcium voltage-gated channel auxiliary subunit alpha 2 delta 1
• trophoblast
• Wnt/beta-catenin

Background and aim: Trophoblasts play an important role in embryo implantation and make a connection with deciduas. Dysregulation of human trophoblast invasion and differentiation can result in pre-eclampsia (PE), a hypertensive disorder of pregnancy with significant morbidity and mortality for mothers and their offspring. In our study, we explored the role of the expression of microRNA-155 (miR-155) in trophoblast invasion and differentiation which is associated with PE pathology. Methods: Trophoblasts were isolated from placentas of patients with PE and the expression of miR-155 and its potential target calcium voltage-gated channel auxiliary subunit alpha 2 delta 1 (CACNA2D1) was assayed by real-time PCR. Computational analysis and luciferase reporter assays were then performed to identify the putative target of miR-155. Next, the proliferation and invasion of the primary trophoblast after transfection of miR-155 mimic were assayed by MTT and Transwell chambers, respectively. Beta-catenin was assayed by Western blot and real-time PCR. Results: The results show that miR-155 inhibits proliferation and cell invasion. Moreover, miR-155 inhibited mRNA and protein expression of beta-catenin and inhibited the proliferation and migration of trophoblasts through Wnt/beta-catenin transcriptional activity. Conclusion: The findings of our study suggest that miR-155 may play a key role in human trophoblast proliferation and invasion, which may contribute to the pathogenesis of PE.

• 出版日期2018
• 单位重庆医科大学