Association of Single Nucleotide Polymorphisms in the IL-18 Gene with Production of IL-18 Protein by Mononuclear Cells from Healthy Donors

作者：Pavlovna Khripko Olga; Sergeevna Sennikova Nadegda; Anatolievna Lopatnikova Julia; Ivanovich Khripko Jurii; Leonidovich Filipenko Maxim; Alexandrovich Khrapov Evgenii; L'vovich Gelfgat Evgenii; Vladimirovna Yakushenko Elena; Alexandrovich Kozlov Vladimir; Vitalievich Sennikov Sergei^*

来源：Mediators of Inflammation, 2008, 2008: 309721.

DOI：10.1155/2008/309721

IL-18 has proinflammatory effects and participates in both innate and adaptive cellular and humoral immunity. A number of SNPs that influence IL-18 production are found in the gene promoter region. We investigated the association of SNPs in the IL-18 promoter at -607 and -137 with the level of IL-18 protein production by PBMC from healthy donors from Southwestern Siberia. The genetic distribution of these SNPs in the promoter site was established by PCR. IL-18 protein production was determined by ELISA. Our results showed that PBMC from donors carrying allele 137C have lower levels of both spontaneous and LPS-stimulated IL-18 production. In contrast, PBMC from donors carrying allele 607A showed significant increases in spontaneous and stimulated IL-18 production compared to wild type. Our study suggests that the SNPs -607 and -137 in the promoter region of the IL-18 gene influence the level of IL-18 protein production by PBMC from healthy donors in Southwestern Siberia.

• 出版日期2008