A Potent Brucella abortus 2308 Delta ery Live Vaccine Allows for the Differentiation between Natural and Vaccinated Infection

作者：Zhang, Junbo; Yin, Shuanghong; Guo, Fei; Meng, Ren; Chen, Chuangfu^*; Zhang, Hui; Li, Zhiqiang; Fu, Qiang; Shi, Huijun; Hu, Shengwei; Ni, Wei; Li, Tiansen; Zhang, Ke

来源：Journal of Microbiology, 2014, 52(8): 681-688.

DOI：10.1007/s12275-014-3689-9

• Brucella abortus 2308
• ery promoter mutant
• live attenuated vaccine
• EryA

Brucellosis is a globally distributed zoonotic disease that causes animal and human diseases. However, the current Brucella abortus vaccines (S19 and RB51) are deficient; they can cause abortion in pregnant animals. Moreover, when the vaccine S19 is used, tests cannot differentiate natural from vaccinated infection. Therefore, a safer and more potent vaccine is needed. A Brucella abortus 2308 ery promoter mutant (Delta ery) was constructed to overcome these drawbacks. The growth of the Delta ery mutant was significantly attenuated in macrophages and mice and induced high protective immunity in mice. Moreover, Delta ery induced an anti-Brucella-specific IgG (immunoglobulin G) response and stimulated the expression of interferon-gamma (INF-gamma) and interleukin-4 (IL-4). Furthermore, the expression of EryA antigen allowed for the serological differentiation between natural and vaccinated infection in mice. These results indicate that the Delta ery mutant is a potential attenuated live vaccine candidate against virulent Brucella abortus 2308 (S2308) infection.

• 出版日期2014-8
• 单位石河子大学