Detection of human adenovirus hexon antigen using carbon nanotube sensors

作者：Jin Xue Hai^*; Ishii Atsushi; Aoki Koki; Ishida Susumu; Mukasa Koichi; Ohno Shigeaki

来源：Journal of Virological Methods, 2011, 171(2): 405-407.

DOI：10.1016/j.jviromet.2010.12.004

• Human adenoviruses
• Carbon nanotube sensor
• Antigen-antibody interaction

Human adenoviruses (HAdVs) have been implicated in a wide range of diseases affecting primarily the respiratory, ocular and gastrointestinal systems. A rapid and efficient method for the detection of HAdV hexon antigen is described using carbon nanotube (CNT) sensors. Anti-HAdV antibody was immobilised on the reverse surface of a CNT sensor. As a control, non-specific mouse IgG was immobilised on another CNT sensor. I-V(gate) curves were measured after incubation of various concentrations of recombinant HAdVs hexon antigen with anti-HAdVs antibody-immobilised or non-specific mouse IgG-immobilised sensors. The curves showed a positive shift that was dependent on the hexon antigen concentration in the anti-HAdV antibody-immobilised sensor, whereas no such shift was observed in the non-specific mouse IgG-immobilised sensor. The sensitivity of the CNT sensor method was greater than that of enzyme-linked immunosorbent assay. Hence, this method offers a new tool for HAdV detection by analysing antigen-antibody interactions.

• 出版日期2011-2