Anti-leishmanial Activities of Extracts and Isolated Compounds from Drechslera rostrata and Eurotium tonpholium

作者：Awaad Amani S^*; Al Zaylaee Haifa M; Alqasoumi Saleh I; Zain Mohamed E; Aloyan Ebtesam M; Alafeefy Ahmed M; Awad Elham S H; El Meligy Reham M

来源：Phytotherapy Research, 2014, 28(5): 774-780.

DOI：10.1002/ptr.5096

• Drechslera rostrata
• Eurotium tonpholium
• ergosterol-5
• 8-peroxide
• diisooctyl phthalate
• 1
• 8-dihydroxy-3-methoxy-6-methyl-anthraquinone
• mannitol
• Leishmania major
• anti-leishmanial activity

The fungal extract of Drechslera rostrata and Eurotium tonpholium showed a significant anti-leishmanial activity against Leishmania major; IC50 was 28.8 and 28.2g/mL, respectively. Seven compounds, five from D. rostrata (H1-H5) and two from E. tonpholium (H6 and H7), were isolated and identified using different spectroscopic analysis including (HNMR)-H-1, (CNMR)-C-13, Hetero-nuclear multiple bond connectivity (HMBC), Hetero-nuclear Multiple Quantum Correlation (HMQC), and EI-MS. The isolated compounds are: di-2-ethylhexyl phthalate (1), (22E)-5,8-epidioxyergosta-6,22-diene-3-ol (2),1,3,8-trihydroxy-6-methyl-nthraquinone (3), aloe-emodine 8-O-glucopyranoside(4), 2R, 3R,4R,5R hexane 1, 2, 3, 4, 5, 6 hexole (Mannitol) (5), 1,8-dihydroxy-3-methoxy-6-methyl-anthraquinone (6) and 1, 4, 5-trihydroxy-7-methoxy-2-methyl-anthraquinone (7). However, compounds (1) and (6) showed activity against L. major with IC50 of 3.2 and 10.38 mu g/mL, respectively. On the other hand, oral administration of the two extracts (100mg/kg) and compounds 1 and 6 (50mg/kg) showed very good activity when compared with the anti-leishmanial drug Pentostam (125mg/kg). Interestingly, the complete heeling activity of the extracts and compounds (1) and (6) was obtained after 13-17days of treatment, while complete healing activity of Pentostam was obtained after 28days. No alteration on liver and kidney functions was recorded on animals treated with the two extracts for 15 consecutive days.

• 出版日期2014-5