Selected hemolymph constituents of captive, biomedically bled, and wild caught adult female American horseshoe crabs (Limulus polyphemus)

作者：James Pirri Mary Jane^*; Veillette Philip A; Leschen Alison S

来源：Marine and Freshwater Behaviour and Physiology, 2012, 45(4): 281-289.

DOI：10.1080/10236244.2012.730216

• Limulus
• biomedical bleeding
• captivity
• hemolymph
• protein

Hemolymph from adult female American horseshoe crabs (Limulus polyphemus) was analyzed from wild caught and three treatments from a biomedical bleeding experiment: captive control, captive bled, and handled according to Best Management Practices (BMP). A total of 10 constituents were measured: blood urea nitrogen, creatinine, glucose, lactate, protein, and ionic concentrations of calcium, chloride, magnesium, potassium, and sodium. Protein concentration was positively correlated with size (prosomal width), while sodium and potassium were negatively correlated with size. Only protein concentration differed among groups, with the captive bled BMP group having significantly lower protein values than either captive control or wild crabs. Wild crabs had higher creatinine, glucose, and potassium values compared to all captive groups. Chloride, calcium, magnesium, and sodium concentrations were lower for wild crabs compared to the captive groups. Lower protein values in the captive bled BMP group suggest that prolonged biomedical bleeding may impact crab physiology.

• 出版日期2012