Antiviral activity of doxycycline against vesicular stomatitis virus in vitro

作者：Wu, Zhuan-chang; Wang, Xin; Wei, Jian-chao; Li, Bei-bei; Shao, Dong-hua; Li, Yu-ming; Liu, Ke; Shi, Yuan-yuan; Zhou, Bin; Qiu, Ya-feng; Ma, Zhi-yong^*

来源：FEMS Microbiology Letters, 2015, 362(22): fnv195.

DOI：10.1093/femsle/fnv195

• doxycycline
• vesicular stomatitis virus
• antiviral activity

Doxycycline (Dox) is a tetracycline derivative with broad-spectrum antimicrobial activities that is used as an effector substance in inducible gene-expression systems. We investigated the antiviral activity of Dox against vesicular stomatitis virus (VSV) infection in cultured H1299 cells. Dox at concentrations of 1.0-2.0 mu g ml(-1) significantly inhibited VSV replication and the VSV-induced cytopathic effect in dose-dependent manners, suggesting that Dox may have broader activity in inhibiting viral replication, in addition to its well-defined bacteriostatic activity. Dox exerted its antiviral effect at the early-mid stage of VSV infection, suggesting that it did not interfere with VSV infectivity, adsorption, or entry into target cells. These results indicate that Dox can inhibit VSV infection and may therefore have potential applications for the treatment of viral infections.

• 出版日期2015-11
• 单位中国农业科学院上海兽医研究所; 南京农业大学