Airway cells from protracted bacterial bronchitis and bronchiectasis share similar gene expression profiles

作者：Chen Alice C H; Pena Olga M; Nel Hendrik J; Yerkovich Stephanie T; Chang Anne B; Baines Katherine J; Gibson Peter G; Petsky Helen L; Pizzutto Susan J; Hodge Sandra; Masters Ian B; Buntain Helen L; Upham John W^*

来源：Pediatric Pulmonology, 2018, 53(5): 575-582.

DOI：10.1002/ppul.23984

• airway and lung cell biology
• bronchiectasis and primary ciliary dyskinesia
• immunology and immunodeficiency
• infections: pneumonia
• TB
• viral
• lung pathology

AimProtracted bacterial bronchitis (PBB) is a common cause of prolonged cough in young children, and may be a precursor of bronchiectasis. Bacteria are often present in the lower airways in both PBB and bronchiectasis and may cause persistent infections. However, there is a paucity of information available on the pathogenesis of PBB and the factors associated with persistent bacterial infection and progression to bronchiectasis. This study hypothesised that lung immune cells in recurrent PBB and bronchiectasis differentially express genes related to immune cell dysfunction compared to lung immune cells from control subjects.
MethodCells isolated from bronchoalveolar lavage (adult-control and PBB BAL cells) were stimulated with nontypeable Haemophilus influenzae (NTHi), and expression of genes involved in various inflammatory pathways was assessed.
ResultNTHi induced production of large amounts of IL-1, IL-6, and IL-8 in adult-control BAL cells, however BAL cells from PBB airways appeared refractory to NTHi stimulation. BAL cells from PBB and bronchiectasis showed differential expression of several genes relative to control cells, including CCL20, MARCO, CCL24, IL-10, PPAR-, CD200R, TREM2, RelB. Expression of genes involved in resolution of inflammation and anti-inflammation response, such as CD200R and IL-10, was associated with the number of pathogenic bacteria found in the airways.
ConclusionIn summary, we have shown that the expression of genes related to macrophage function and resolution of inflammation are similar in PBB and bronchiectasis. Lung immune cell dysfunction in PBB and bronchiectasis may contribute to poor bacterial clearance and prolonged resolution of inflammation.

• 出版日期2018-5