摘要

A mircrofluidic chip, based on using an air stream for fluidic controlling and coupled with chemiluminescence (CL) detection, was presented. The reported design promised a simple, low cost chip fabrication, and an easy operation. With each assay time less than 3 min, the described microfluidic chip was applied in determination of iron(II) content in nature water and total iron content in whole blood. Experiment results showed the potential for a multitude of applications of the presented chip. The calibration graph of chemiluminescent intensity (I-CL) vs. iron(II) concentration was linear in the range 1.0 x 10(-6) -5.0 x 10(-)5 mol L-1, and the detection limit (DL) was 3.0 x 10(-7) mol L-1 (3sigma).