A novel xenograft model with intrinsic vascularisation for growing undifferentiated pleomorphic sarcoma NOS in mice

作者：Tilkorn Daniel Johannes^*; Daigeler Adrien; Hauser Joerg; Ring Andrej; Stricker Ingo; Schmitz Inge; Steinstraesser Lars; Steinau Hans Ulrich; Al Benna Sammy

来源：Journal of Cancer Research and Clinical Oncology, 2012, 138(5): 877-884.

DOI：10.1007/s00432-012-1163-9

• Pleomorhic sarcoma NOS
• Athymic mice
• Xenograft model
• Soft tissue sarcoma

Preclinical development of antisarcoma therapy is primarily based on the subcutaneous transplantation of sarcoma xenografts. Tumour cell survival remains a hurdle of current models, which has been attributed to the hypoxic conditions following transplantation. We hypothesised that sarcoma models with an intrinsic tissue-engineered vascular supply are easily reproducible. The aim of this study was to establish a novel vascularised xenograft model. %26lt;br%26gt;Primary human soft tissue sarcomas were transplanted into a silicon chamber and placed around the superficial epigastric vessels of nude mice. Sarcoma xenograft samples were assessed histomorphologically. %26lt;br%26gt;All sarcoma xenografts engrafted, leading to solid tumours. Histological, immunohistochemical staining and light/electron microscopy confirmed the xenografts as identical high-grade pleomorphic sarcomas (NOS) compared with the original patients%26apos; tumours. %26lt;br%26gt;This novel sarcoma xenograft model with an intrinsic vascular supply could be of high value for studying human soft tissue sarcomas and their therapy.