A subtle calculation method for nanoparticle's molar extinction coefficient: The gift from discrete protein-nanoparticle system on agarose gel electrophoresis

作者：Zhong, Ruibo; Yuan, Ming; Gao, Haiyang; Bai, Zhijun; Guo, Jun; Zhao, Xinmin; Zhang, Feng^*

来源：Functional Materials Letters, 2016, 9(2): 1650029.

DOI：10.1142/S1793604716500296

• Gold nanoparticle
• BSA
• discrete conjugate
• molar ratio
• spectroscopy
• ultracentrifuge

Discrete biomolecule-nanoparticle (NP) conjugates play paramount roles in nanofabrication, in which the key is to get the precise molar extinction coefficient of NPs. By making best use of the gift from a specific separation phenomenon of agarose gel electrophoresis (GE), amphiphilic polymer coated NP with exact number of bovine serum albumin (BSA) proteins can be extracted and further experimentally employed to precisely calculate the molar extinction coefficient of the NPs. This method could further benefit the evaluation and extraction of any other dual-component NP-containing bio-conjugates.

• 出版日期2016-4
• 单位内蒙古农业大学