Morphometric and high resolution scanning electron microscopy analysis of low-level laser therapy and latex protein (Hevea brasiliensis) administration following a crush injury of the sciatic nerve in rats

作者：Dias Fernando J; Issa Joao Paulo M; Coutinho Netto Joaquim; Fazan Valeria P S; Sousa Luiz Gustavo; Iyomasa Mamie M; Papa Paula C; Watanabe Ii sei^*

来源：Journal of the Neurological Sciences, 2015, 349(1-2): 129-137.

DOI：10.1016/j.jns.2014.12.043

• Nerve regeneration
• Low-level laser therapy
• Latex protein-F1
• Morphometric
• Ultrastructure
• High-resolution scanning electron microscopy

This study evaluated the effect of low-level laser therapy (LLLT; 15 J/cm(2)) and a latex protein (F1) on a crush injury of the sciatic (ischiadicus) nerve. Seventy-two rats (male, 250 g) were divided into 6 groups: CG, control; EG, exposed nerve; IG, injured nerve without treatment; LG, injured nerve with LLLT; HG, injured nerve with F1; and LHG, injured nerve with LLLT and F1. After 4 or 8 weeks, the animals were euthanized and samples of the sciatic nerve were collected for morphometric and high-resolution scanning electron microscopy (HRSEM) analysis. After 4 weeks, the morphometry revealed improvements in the treated animals, and the HG appeared to be the most similar to the CG; after 8 weeks, the injured groups showed improvements compared to the previous period, and the results of the treatment groups were more similar to one another. At HRSEM after 4 weeks, the treated groups were similar and showed improvement compared to the IG; after 8 weeks, the LHG and HG had the best results. In conclusion, the treatments resulted in improvement after the nerve injury, and this recovery was time-dependent. In addition, the use of the F1 resulted in the best morphometric and ultrastructural findings.

• 出版日期2015-2-15