A TICT based two-photon fluorescent probe for bisulfite anion and its application in living cells

作者：Yu, Shenglong; Yang, Xiuli; Shao, Zhonglong; Feng, Yan; Xi, Xinguo; Shao, Rong; Guo, Qingxiang; Meng, Xiangming^*

来源：Sensors and Actuators B: Chemical , 2016, 235: 362-369.

DOI：10.1016/j.snb.2016.05.099

• TICT
• Two-photon
• Fluorescent probe
• Coumarin
• Bisulfite anion

A two-photon fluorescent probe (DMPCA) for the detection of bisulfite anion in water was developed based on twisted-intramolecular-charge-transfer (TICT) mechanism. Fluorescence intensity of DMPCA increased dramatically (65 fold) upon the addition of HS O-3(-). The two-photon absorption cross-section of DMPCA was found to be 725 GM at 700 nm upon the addition of HSO3-. The fluorescent probe showed an excellent selectivity for bisulfite anion over other anions. The nucleophilic addition reaction mechanism with aldehyde was confirmed with NMR and MALDI-TOF. The TICT detection mechanism was confirmed by viscosity-dependent fluorescence spectra and time resolved fluorescence spectra. Moreover, DMPCA was successfully applied to the detection of bisulfite anion in HeLa cells with low cytotoxicity under two-photon excitation.

• 出版日期2016-11-1
• 单位盐城工学院; 安徽大学; 中国科学技术大学