Membrane remodeling, an early event in benzo[a]pyrene-induced apoptosis

作者：Tekpli Xavier; Rissel Mary; Huc Laurence; Catheline Daniel; Sergent Odile; Rioux Vincent; Legrand Philippe; Holme Jorn A; Dimanche Boitrel Marie Therese; Lagadic Gossmann Dominique^*

来源：Toxicology and Applied Pharmacology, 2010, 243(1): 68-76.

DOI：10.1016/j.taap.2009.11.014

• Cholesterol-rich microdomains
• Aryl hydrocarbon receptor
• HMG-CoA reductase
• Reactive oxygen species
• Apoptosis

Benzo[a]pyrene (B[a]P) often serves as a model for mutagenic and carcinogenic polycyclic aromatic hydrocarbons (PAHs). Our previous work suggested a role of membrane fluidity in B[a]P-induced apoptotic process. In this study, we report that B[a]P modifies the composition of cholesterol-rich microdomains (lipid rafts) in rat liver F258 epithelial cells. The cellular distribution of the ganglioside-GM1 was markedly changed following B[a]P exposure. B[a]P also modified fatty acid composition and decreased the cholesterol content of cholesterol-rich microdomains. B[a]P-induced depletion of cholesterol in lipid rafts was linked to a reduced expression of 3-hydroxy-3-methylglutaryl-CoA reductase (HMG-CoA reductase). Aryl hydrocarbon receptor (AhR) and B[a]P-related H(2)O(2) formation were involved in the reduced expression of HMG-CoA reductase and in the remodeling of membrane microdomains. The B[a]P-induced membrane remodeling resulted in an intracellular alkalinization observed during the early phase of apoptosis. In conclusion, B[a]P altered the composition of plasma membrane microstructures through AhR and H(2)O(2) dependent-regulation of lipid biosynthesis. In F258 cells, the B[a]P-induced membrane remodeling was identified as an early apoptotic event leading to an intracellular alkalinization.

• 出版日期2010-2-15