Astragalus mongholicus polysaccharide inhibits lipopolysaccharide-induced production of TNF-alpha and interleukin-8

作者：Yuan Yuan; Sun Mei; Li Ke Shen

来源：World Journal of Gastroenterology, 2009, 15(29): 3676-3680.

DOI：10.3748/wjg.15.3676

• Astragalus mongholicus polysaccharide
• Intestinal epithelial cells
• Tumor necrosis factor-alpha
• Interleukin-8
• Extracellular signal-regulated kinase
• C Jun amino-terminal kinase
• p38 kinase

AIM: To explore the effect of Astragalus mongholicus polysaccharide (APS) on gene expression and mitogen-activated protein kinase (MAPK) transcriptional activity in intestinal epithelial cells (IEC).
METHODS: IEC were divided into control group, lipopolysaccharide (LPS) group, LPS 50 mu g/mL APS group, LPS 100 mu g/ml APS group, LPS 200 mu g/mL APS group, and LPS 500 mu g/mL APS group. Levels of mRNAs in LPS-induced inflammatory factors, tumor necrosis factor (TNF)-alpha and interleukin (IL)-8, were measured by reverse transcription-polymerase chain reaction. MAPK protein level was measured by Western blotting.
RESULTS: The levels of TNF-alpha and IL-8 mRNAs were significantly higher in IEC with LPS-induced damage than in control cells. APS significantly abrogated the LPS-induced expression of the TNF-alpha and IL-8 genes. APS did not block the activation of extracellular signal-regulated kinase or c Jun amino-terminal kinase, but inhibited the activation of p38, suggesting that APS inhibits LPS-induced production of TNF-alpha and IL-8 mRNAs, possibly by suppressing the p38 signaling pathway.
CONCLUSION: APS-modulated bacterial product-mediated p38 signaling represents an attractive strategy for prevention and treatment of intestinal inflammation.

• 出版日期2009-8-7
• 单位中国人民解放军第二一一医院; 哈尔滨工程大学; 中国医科大学