Determination of Tetracycline in Pharmaceutical Preparation by Molecular and Atomic Absorption Spectrophotometry and High Performance Liquid Chromatography via Complex Formation with Au(III) and Hg(II) Ions in Solutions

作者：Abdulghani Ahlam Jameel^*; Jasim Hadi Hassan; Hassan Abbas Shebeeb

来源：International Journal of Analytical Chemistry, 2013, 2013: 305124.

DOI：10.1155/2013/305124

UV-visible and atomic spectrophotometry and HPLC techniques were applied for the determination of tetracycline (TC) in pharmaceutical preparations via complexation of the drug with Au(III) and Hg(II) ions in solutions. The mole ratio of TC to metal ions was 1 : 1. Maximum peak absorption at lambda 425 and 320 nm for the two ions, respectively, was optimized at heating temperature 75 degrees C for 15 minutes at pH = 4 followed by the extraction with ethyl acetate. The percentage of extraction and stability constants for the two complexes was 95.247, 95.335% and 2.518 x 10(4), 1.162 x 10(5) M-1, respectively. HPLC method was applied without extraction process. The analytical data obtained from direct calibration curves of UV-visible absorption, FAAS, and HPLC for Au(III) complexes were recovery (100.78, 104.85, and 101.777%, resp.); detection limits (0.7403, 0.0997, and 2.647 mu g/ml, resp.); linearity (5-70, 5-30, and 10-150 mu g/ml, resp.), and correlation coefficient (0.9991, 0.9967, and 0.9986, resp.). The analytical data obtained from direct calibration curves for Hg(II) complexes by UV-visible spectrophotometry and HPLC were recovery (100.95 and 102.000%, resp.); detection limits (0.5867 and 2.532 mu g/ml, resp.); linearity (5-70 and 10-150 mu g/ml, resp.); and correlation coefficients (0.9989 and 0.9997, resp.).

• 出版日期2013