An Efficient Method for Detection of Recombinant Proteins Using a Bacteriophytochrome Chromophore Binding Domain from Deinococcus Radiodurans

作者：Yoon Joo Mi; Hahn Bum Soo; Kim Tae Lim; Cho Man Ho; Bhoo Seong Hee; Hahn Tae Ryong; Lee Sang Won^*

来源：Journal of the Korean Society for Applied Biological Chemistry, 2013, 56(1): 103-106.

DOI：10.1007/s13765-012-2225-z

• bacteriophytochrome
• Deinocuccus radiodurans
• fusion protein technology
• protein purification

Bacteriophytochromes are composed of N-terminal region for chromophore binding and C-terminal domain for transmission of a light signal. In this study, the possibility of using the chromophore-binding domain from a Deinococcus radiodurans bacteriophytochrome as a protein tag to enable a rapid and simple detection method for the production of recombinant proteins were evaluated.

• 出版日期2013-2