Massively multiplex single-cell Hi-C

作者：Ramani, Vijay; Deng, Xinxian; Qiu, Ruolan; Gunderson, Kevin L.; Steemers, Frank J.; Disteche, Christine M.; Noble, William S.; Duan, Zhijun^*; Shendure, Jay^*

来源：Nature Methods, 2017, 14(3): 263-+.

DOI：10.1038/NMETH.4155

• PROXIMITY LIGATION
• HUMAN GENOME
• CHROMOSOME
• ORGANIZATION
• ARCHITECTURE
• PRINCIPLES
• LINE

We present single-cell combinatorial indexed Hi-C (sciHi-C), a method that applies combinatorial cellular indexing to chromosome conformation capture. In this proof of concept, we generate and sequence six sciHi-C libraries comprising a total of 10,696 single cells. We use sciHi-C data to separate cells by karyotypic and cell-cycle state differences and identify cell-to-cell heterogeneity in mammalian chromosomal conformation. Our results demonstrate that combinatorial indexing is a generalizable strategy for single-cell genomics.

• 出版日期2017-3