科研之友
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摘要
Triphenylmethane dyes are aromatic xenobiotic compounds that are widely considered to be one of the most recalcitrant pollutants discharged into the environment. A fungus Penicillium sp. having decolorization capacities for Malachite Green was isolated from contaminated soil, given name YW 01. Plackett-Burman and Box-Behnken designs were used to screen the effects of decolorization factors and obtain the optimum conditions for decolorization of Malachite Green, respectively. Sucrose concentration had a significantly negative effect on the decolorization of Malachite Green, whereas the effects of initial pH and inoculum size were significantly positive (p < 0.05). Optimum conditions by Box-Behnken design for the decolorization process were sucrose concentration 9.02 g/L, initial pH 6.0, and inoculum size 3.5 g (wet weight). 98.23% decolorization was observed for 25 mg/L dye concentration after 6 days under optimum conditions, which was close to the predicted value 98.93%. Induction in the activities of lignin peroxidase and NADH-dependent 2,6-dichlorophenolindophenol reductase was observed during Malachite Green decolorization, indicating these enzymes involved in the decolorization process. Information from pseudo-first-order kinetic function for decolorization process of Malachite Green (R(2) = 0.9221) implied the decolorization half-life of Malachite Green by Penicillium sp. YW 01 was 0.92 day, indicating that Penicillium sp. YW 01 was efficient for the degradation of Malachite Green and useful for the biological treatment of dye effluents.
- 出版日期2011-8
- 单位浙江大学
