Direct detection of human herpesvirus 6B by the LAMP method using newly developed dry-reagents

作者：Yoshikawa Tetsushi^*; Matsuo Takahiro; Kawamura Yoshiki; Ohashi Masahiro; Yonekawa Toshihiro; Kanda Hidetoshi; Notomi Tsugunori; Ihira Masaru

来源：Journal of Virological Methods, 2014, 201: 65-67.

DOI：10.1016/j.jviromet.2014.02.017

• HHV-6B
• LAMP
• Dry-reagent
• Rapid diagnosis
• Exanthem subitum

The reliability of the HHV-6B LAMP using the dry-reagent method was evaluated using serum samples obtained from febrile children. The sensitivity of the original and dry-reagent methods was 10 copies/reaction and 100 copies/reaction, respectively. The dry-reagent LAMP method was highly sensitive (94.0%) and specific (96.0%) for the detection of HHV-6B.

• 出版日期2014-6-1