Development of Transformation System of Verticillium lecanii (Lecanicillium spp.) (Deuteromycotina: Hyphomycetes) Based on Nitrate Reductase Gene of Aspergillus nidulans

作者：Hasan Saba^*; Singh Rana Inder; Singh Sandhu Sardul

来源：Indian Journal of Microbiology, 2011, 51(3): 390-395.

DOI：10.1007/s12088-011-0106-5

• Verticillium lecanii
• Nitrate reductase
• Heterologous transformation
• Plasmid pBT

A heterologous transformation system was developed for V. lecanii based on the complementation of a nitrate reductase mutant. Nitrate reductase mutants were obtained by resistance to chlorate in a rate of 23.24% when compared to other mutations that lead to the chlorate resistance. Mutant no. 01 and 04 was chosen for the transformation experiments. Plasmid pBT was used as transformation vector containing the Aspergillus nidulans nitrate reductase gene. A frequency of approximately 3 transformants/mu g DNA was obtained using the circular vector pBT. The establishment of a transformation system for V. lecanii is fundamental for genetic manipulation of this microorganism.

• 出版日期2011-7