Antibiotic furazolidone induces CYP1A but not CYP2E1 subfamily in rat liver

作者：Sasaki Nobuo; Sakai Noriaki; Ikenaka Yoshinori; Kamiya Tomomi; Heewon Min; Sakamotoo Kentaro Q; Ishizuka Mayumi; Fujita Shoichi^*

来源：Journal of Veterinary Medical Science, 2008, 70(3): 223-226.

DOI：10.1292/jvms.70.223

• CYP1A1
• cytochrome P450
• furazolidone
• mutagenicity
• rat

Furazolidone (FZ), one of the nitrofuran fungicides, is used as a veterinary medicine in the Middle and Far Eastern countries. In this study, FZ (125 mg/kg) was administered orally to Wistar rats for 3 days. Results of the Ames test using the S-9 fraction of rats treated with FZ showed a significant increase in the number of revertant colonies. Western blot analysis of hepatic CYP isozymes induced by FZ, revealed a remarkable induction of CYP1A1 apoprotein, but CYP1A2 and CYP2E1 apoproteins were not altered. In addition, the expression of CYP1A1 mRNA level in rats treated with FZ by RT-PCR was significantly enhanced by FZ treatment. We concluded that FZ is apparently mutagenic and induces transcription of the CYP1A1 isozyme, which metabolically activates numerous promutagens, in hepatocytes.

• 出版日期2008-3