Abundant Variation of Waxy Gene in Yunnan Rice Landraces and Molecular Characterization of a Novel Wx(zm) Allele

作者：Chen, Hong; Shan, Jia; Yang, Kun; Wang, Yun-Yue; Lu, Chun-Ming^*

来源：Crop Science, 2014, 54(5): 2152-2159.

DOI：10.2135/cropsci2013.08.0548

• ORYZA-SATIVA L.
• AMYLOSE CONTENT
• GLUTINOUS RICE
• ENDOSPERM
• WX
• EXPRESSION
• GERMPLASM

Waxy is the key gene that controls the apparent amylose content (AC) in rice (Oryza sativa L.) endosperm. In this study three allele-specific polymerase chain reaction (AS-PCR) primer sets for Wx(mq) and Wx(op/hp) were developed that provided new markers for determining and selecting targeted Waxy alleles. The AC determination and AS-PCR results indicated abundant AC variation and extremely rich Waxy allelic diversity in Yunnan rice landraces such that 70 Waxy alleles were detected in 404 landraces. For non-glutinous rice, association analysis showed that only two of the seven Waxy functional single nucleotide polymorphisms (SNPs) were significantly correlated to AC variance. The intron 1 splice site (In1) explained 3.45% and all seven sites explained 9.79% of the total AC variance, respectively, which were much less compared to previous studies. A novel Wx(zm) allele with a nonsynonymous C to T transition at E10(ZM) site located at 2134 bp of exon 10 was characterized from a glutinous landrace Zimi. This transition led to a Leu to Phe substitution at the glycosyl transferases domain of granule-bound starch synthase I (GBSSI), which was highly conserved in Poaceae crops. Protein analysis showed that Zimi had similar endosperm GBSSI accumulation as other nonglutinous landraces but its activity was dramatically decreased, indicating that this Leu residue was critical for the glycosyl transfer activity of GBSSI. The characterization of Wx(zm) revealed a new mechanism for rice glutinous formation and suggested multiple origins of the rice glutinous phenotype.

• 出版日期2014-10
• 单位云南农业大学