A hevein-like protein and a class I chitinase with antifungal activity from leaves of the paper mulberry

作者：Zhao, Ming; Ma, Yan; Pan, Ying-Hong^*; Zhang, Chun-Hua; Yuan, Wen-Xia

来源：Biomedical Chromatography, 2011, 25(8): 908-912.

DOI：10.1002/bmc.1543

• antifungal protein
• fast protein liquid chromatography
• matrix-assisted laser desorption ionization time-of-flight mass
• spectrometry
• peptide mapping fingerprint analysis

Paper mulberry (Broussonetia papyrifera, syn. Morus papyrifera L.) is a Chinese traditional medicine and its low-molecular-weight extracts are reported to have antifungal activity. In this study, two proteins (PMAPI and PMAPII) with activity against Trichoderma viride were obtained from paper mulberry leaves with a fast protein liquid chromatography (FPLC) unit. The purification protocol employed (NH(4))(2)SO(4) precipitation, ion-exchange chromatography and hydrophobic-interaction chromatography on FPLC. Molecular masses were 18,798 Da for PMAPI, and 31,178 Da for PMAPII determined by Matrix-assisted laser desorption ionization time-of-flight mass spectrometry. Peptide mapping fingerprint analysis showed that PMAPI has no peptides similar to PMAPII. N-terminal amino acid sequencing revealed that PMAPI is a hevein-like protein, and PMAPII is a class I chitinase. They both had a half-maximal inhibitory concentration (IC50) of 0.1 mu g/mu L against T. viride. This is the first report of high-molecular-weight extracts with antifungal activity from paper mulberry.

• 出版日期2011-8
• 单位中国农业科学院; 云南农业大学