Down-regulation of miR-141 in gastric cancer and its involvement in cell growth

作者：Du, Ying; Xu, Yanjun; Ding, Ling; Yao, Haomi; Yu, Hong; Zhou, Tianhua; Si, Jianmin^*

来源：Journal of Gastroenterology, 2009, 44(6): 556-561.

DOI：10.1007/s00535-009-0037-7

• MiR-141
• Gastric cancer
• MGC-803 cell
• Cell proliferation

Human microRNA-141 (miR-141), a member of the miR-200 family, has been reported to be associated with various human malignancies. However, it remains unknown whether miR-141 is involved in the pathogenesis of gastric cancer. Therefore, we examined the expression of miR-141 in gastric cancer tissues and the effect of miR-141 overexpression on cancer cell proliferation. @@@ The expression level of miR-141 in 35 pair-matched gastric neoplastic and adjacent non-neoplastic tissues, and in 5 gastric cancer cell lines were examined by quantitative real-time PCR. The growth of MGC-803 cells transfected with miRNA precursor was examined by MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl-tetrazoliumbromide) assay. @@@ MiR-141 was significantly down-regulated in 80% (28/35) of primary gastric cancer tissues compared with pair-matched adjacent non-tumor tissues (P < 0.01). The expression of miR-141 was also found to be substantially reduced in several human gastric cancer cell lines such as MGC-803, HGC-27, SGC-7901 and BGC-823 cells. Overexpression of miR-141 with its precursors significantly inhibited the proliferation of gastric cancer cells. @@@ These results suggest that miR-141 may be involved in the development of gastric cancer through its inhibitory effect on cell proliferation.

• 出版日期2009-6
• 单位浙江大学