Determination of phenolic compounds and antioxidant activity of a Mediterranean plant: The case of Satureja montana subsp kitaibelii

作者：Lopez Cobo Ana; Maria Gomez Caravaca Ana; Svarc Gajic Jaroslava; Segura Carretero Antonio^*; Fernandez Gutierrez Alberto

来源：Journal of Functional Foods, 2015, 18: 1167-1178.

DOI：10.1016/j.jff.2014.10.023

• Satureja montana subsp kitaibelii
• HPLC-DAD-ESI-TOF-MS
• Antioxidant activity
• Quantification
• Total phenolic compounds

Satureja montana subsp. kitaibelii extract was analyzed by HPLC-DAD-ESI-TOF-MS (high performance liquid chromatography-diode array detector-electrospray ionization-time of flight-mass spectroscopy). Forty five compounds were identified, 42 of which were identified for the first time in this plant. Quantification of the identified phenolic compounds by UV-vis (ultraviolet-visible) detection was done and chlorogenic acid was the most concentrated compound. Total phenolic content (TPC) by Folin-Ciocalteu method and antioxidant activity by DPPH and ABTS methods were also measured. TPC quantified by HPLC-DAD were 1958.44 mg/100 g dry sample. This is in agreement with TPC measured by Folin-Ciocalteu method. Antioxidant activity determined by the DPPH method expressed as EC50 was 116.36 +/- 12.83 mu g/mL and by the ABTS method was 106.59 +/- 1.41 mu mol trolox equivalents/g dry sample. These results reveal that Satureja montana subsp. kitaibelii could be a good functional food ingredient because its high content of natural antioxidants as demonstrated by quantification with HPLC-DAD.

• 出版日期2015-10