Preparation of stable Pd nanoparticles with tunable size for multiple immunolabeling in biomedicine

作者：Slouf Miroslav^*; Pavlova Ewa; Bhardwaj Monika; Plestil Josef; Onderkova Heidemarie; Philimonenko Anatoly A; Hozak Pavel

来源：Materials Letters, 2011, 65(8): 1197-1200.

DOI：10.1016/j.matlet.2011.01.046

• Palladium nanoparticles
• Nanocrystalline materials
• Immunolabeling

Stable Pd nanoparticles (PdNP) with a tunable size (3-15 nm) were synthesized by controlled chemical reduction of PdCl(2) with sodium citrate in water. The morphology of PdNP was characterized by transmission electron microscopy, while their stability in solution was verified by quasi-elastic light scattering and small-angle X-ray scattering. Intensive stirring of reacting mixture played a vital role in achieving reproducible particle sizes. Controlled changes of pH and initial concentrations were employed in fine-tuning particle size distributions. Finally, 10 nm PdNP were conjugated with goat anti-mouse IgG antibody as proved by electrophoresis (SOS-PAGE) and used for ultrastructural immunolabeling, which confirmed suitability of PdNP for multiple immunolabeling in biomedicine.

• 出版日期2011-4-30