Molecular Strategy for Survival at a Critical High Temperature in Eschierichia coli

作者：Murata Masayuki^*; Fujimoto Hiroko; Nishimura Kaori; Charoensuk Kannikar; Nagamitsu Hiroshi; Raina Satish; Kosaka Tomoyuki; Oshima Taku; Ogasawara Naotake; Yamada Mamoru

来源：PLos One, 2011, 6(6): e20063.

DOI：10.1371/journal.pone.0020063

The molecular mechanism supporting survival at a critical high temperature (CHT) in Escherichia coli was investigated. Genome-wide screening with a single-gene knockout library provided a list of genes indispensable for growth at 47 degrees C, called thermotolerant genes. Genes for which expression was affected by exposure to CHT were identified by DNA chip analysis. Unexpectedly, the former contents did not overlap with the latter except for dnaJ and dnaK, indicating that a specific set of non- heat shock genes is required for the organism to survive under such a severe condition. More than half of the mutants of the thermotolerant genes were found to be sensitive to H2O2 at 30 degrees C, suggesting that the mechanism of thermotolerance partially overlaps with that of oxidative stress resistance. Their encoded enzymes or proteins are related to outer membrane organization, DNA double-strand break repair, tRNA modification, protein quality control, translation control or cell division. DNA chip analyses of essential genes suggest that many of the genes encoding ribosomal proteins are down-regulated at CHT. Bioinformatics analysis and comparison with the genomic information of other microbes suggest that E. coli possesses several systems for survival at CHT. This analysis allows us to speculate that a lipopolysaccharide biosynthesis system for outer membrane organization and a sulfur-relay system for tRNA modification have been acquired by horizontal gene transfer.

• 出版日期2011-6-10