DECREASED EXPRESSION OF MATRIX METALLOPROTEINASE-9 AND INCREASED EXPRESSION OF TISSUE INHIBITORS OF MATRIX METALLOPROTEINASE-1 IN PARATUBERCULOSIS-INFECTED CATTLE IN THE ELISA-NEGATIVE SUBCLINICAL STAGE

作者：Wang Xiaofei; Wang Hongyu; Aodon geril; Shu Yujing; Momotani Yuriko; Nagata Reiko; Mori Yasuyuki; Momotani Eiichi^*

来源：Animal Biotechnology, 2011, 22(1): 44-49.

DOI：10.1080/10495398.2010.536096

• Cattle
• Mmp-9
• Mycobacterium
• Paratuberculosis
• Timp-1

We investigated the gene expression of matrix metalloproteinases-9 (MMP-9) and tissue inhibitors of matrix metalloproteinases-1 (TIMP-1) in peripheral blood cells from infected cattle with Mycobacterium avium subsp. paratuberculosis (Map) in the ELISA-negative subclinical stage compared with uninfected control cattle. Significant decreased MMP-9 expression and increased TIMP-1 expression were found in peripheral blood cells from Map-infected cattle after stimulation with Map lysate and Map purified protein derivative (PPD) than in control cattle by real-time RT-PCR analysis. In contrast to the uninfected controls, the activity of MMP-9 was also decreased in peripheral blood cell culture supernatants from Map-infected cattle at 24 hr after Map lysate and MapPPD stimulation by gelatin zymography analysis. As a result, the MMP-9 may play an important role in the development of Mycobacterium avium subsp. paratuberculosis disease.

• 出版日期2011