Diallyl trisulfide and diallyl disulfide ameliorate cardiac dysfunction by suppressing apoptotic and enhancing survival pathways in experimental diabetic rats

作者：Huang Yao Te; Yao Chun Hsu; Way Chia Li; Lee Kung Wei; Tsai Cheng Yen; Ou Hsiu Chung; Kuo Wei Wen^*

来源：Journal of Applied Physiology, 2013, 114(3): 402-410.

DOI：10.1152/japplphysiol.00672.2012

• diabetic cardiomyopathy
• garlic
• PI3k-Akt
• apoptosis
• echocardiography

Huang Y-T, Yao C-H, Way C-L, Lee K-W, Tsai C-Y, Ou H-C, Kuo W-W. Diallyl trisulfide and diallyl disulfide ameliorate cardiac dysfunction by suppressing apoptotic and enhancing survival pathways in experimental diabetic rats. J Appl Physiol 114: 402-410, 2013. First published November 8, 2012; doi:10.1152/japplphysiol.00672.2012.-Cardiovascular disease is one of the major causes of mortality in diabetic patients. Mounting studies have shown that garlic exhibits, possibly through its antioxidant potential, diverse biological activities. In this study, we investigated the alleviating effects of garlic oil (GO) and its two major components, diallyl disulfide (DADS) and diallyl trisulfide (DATS), on diabetic cardiomyopathy in rats. Physiological cardiac parameters were obtained using echocardiography. Apoptotic cells were evaluated using TUNEL and DAPI staining. Protein expression levels were determined using Western blotting analysis. Our findings indicated that in diabetic rat hearts significantly decreased fractional shortening percentage, increased levels of nitrotyrosine, an elevated number of TUNEL-positive cells, enhanced levels of caspase 3 expression, and decreased PI3K-Akt signaling pathway activities were observed. Furthermore, all of these alterations were reversed following both GO and DATS (or DADS) administrations through increasing PI3K-Akt signaling pathway activities and inhibiting both the death receptor-dependent and the mitochondria-dependent apoptotic pathways. In conclusion, this study shows that DATS and DADS, with the efficacy order DATS %26gt; DADS, have the therapeutic potential for ameliorating diabetic cardiomyopathy. Furthermore, the therapeutic effects of GO on diabetic cardiomyopathy should be mainly from DATS and DADS.

• 出版日期2013-2
• 单位中国医科大学